ANUP inhibits KS cell migration. Migration assays were performed in double-chamber wells separated by a fibronectin-coated membrane. Chemotaxis was induced by addition bFGF (25 ng/mL) to the lower chamber. Cells (5 × 104/mL) were placed in the upper chamber in the presence and absence of test compounds. Taxol 10 ng/mL was used as a positive control. Migration of (A) endothelial cells or (B) KS cells across the membrane was quantified after overnight incubation. Representative photographs (original magnification ×360) for controls, various concentrations of ANUP, and taxol are shown for (C) endothelial cells and (D) KS Y-1 cells (see page 1039). Cells that migrated to the underside of the transwell membrane are shown; cells that did not migrate were removed from the upper surface of the membrane with a cotton swab prior to microscopy.
