Fig. 7.
![Fig. 7. Immunoprecipitation analysis of GPIIb-IIIa complexes from CHO cells transiently cotransfected with cDNAs encoding GPIIIa and either normal or [Arg674]GPIIb. CHO cells were transiently transfected with cDNAs encoding GPIIIa and either normal or [Arg674]GPIIb. Surface (upper panel) or total (surface and intracellular, lower panel) labeling of cells was performed with biotin-NHS and the GPIIb-IIIa complexes were immunoprecipitated with anti-GPIIIa (P37) or anti-GPIIb (M3) MoAbs and processed as described in Materials and Methods.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/93/3/10.1182_blood.v93.3.866/5/blod40311007w.jpeg?Expires=1735830607&Signature=aiEgHS6VaIPfwFwWMT2z4raHr7uRnBQa7rE5kL4D53wHY9I00Sz27qhKKNztJqAN3clbZ2MGKB2fx1z73OGbrrXKf7gVzKE8ZPP5u5kggrcM2810M-T9w0pTe7dgbiX~55Z0daqROe7zrRldAgXdBZuBNrfurZG76IbZH7hzPSxoE18Ms547zWG8qx4orFL-lPhDY9R~aANoaUB0L2lR0mtBr-UcpSLvUQqcysyshu5YRlNvYu9bFwd1L2N-NySgNE7vSl9GR8Qvd4css0Z-U2Gvue4aqUzDIh5aKAhoK04kpyOB9KBBLQclOfMuDjqZFxC5pqKZKSbsphyYNRDBEg__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Immunoprecipitation analysis of GPIIb-IIIa complexes from CHO cells transiently cotransfected with cDNAs encoding GPIIIa and either normal or [Arg674]GPIIb. CHO cells were transiently transfected with cDNAs encoding GPIIIa and either normal or [Arg674]GPIIb. Surface (upper panel) or total (surface and intracellular, lower panel) labeling of cells was performed with biotin-NHS and the GPIIb-IIIa complexes were immunoprecipitated with anti-GPIIIa (P37) or anti-GPIIb (M3) MoAbs and processed as described in Materials and Methods.
