Effects of antisense p21 mRNA expression on centrosome distribution. These three pictures show examples of cells containing nuclei and stained in situ with antibody to γ-tubulin, which localizes the centrosome (red foci). The nuclei are visualized with DAPI (blue). The insets are phase contrast images of the same cells stained with Giemsa to show the cellular boundries. The upper picture shows three cells. The upper two cells are mononuclear cells and demonstrate a single red centrosomal focus (see text for further descriptions). The lower cell contains a deformed nucleus and demonstrates a cluster of several centrosomal foci. This type of cell was found exclusively in AS21 cultures. The other two pictures show further examples of cells with deformed nuclei also containing several centrosomal foci each.