Schematic representation of ETV6, TRKC, and predicted ETV6-TRKC protein. Extracellular and transmembrane domain of TRKC is replaced by PNT domain of ETV6 as the result of t(12;15). C-1 chimeric transcript encodes the complete sequence of the PTK domain, but in C-2 and C-3, the 3′-terminal 462 bp of TRKC is truncated within the PTK domain. Nucleic acid and amino acid sequences surrounding the breakpoint are shown beneath. Vertical arrows indicate the breakpoint. Primers used for RT-PCR are indicated with small arrows. SP, putative signal peptide; TM, transmembrane domain; PTK, protein-tyrosine kinase domain; PNT, pointed domain; ETS, ETS binding domain.