RT-PCR analysis of ETV6-TRKC fusion products. Total RNA from t(12;15) leukemic cells (lanes 1 through 4) or NBTM cell line (lane 5) was reverse transcribed. PCR amplification was performed using primers ETV6-116f and ETV6-575r (lane 1),ETV6-116f and TRKC-1947r (lane 2), TRKC-1556f and ETV6-575r (lane 3), and TRKC-1556f andTRKC-1947r (lanes 4 and 5), respectively.