Effects of CD437 on NB4 cell viability, apoptosis, and DEVD-amc hydrolytic activity. (A) NB4 cells (300,000/mL) were treated for the indicated amount of time with vehicle (DMSO, open symbols) or with 10−6 mol/L CD437 (solid symbols). Circles indicate the percentage of viable cells (erythrosin staining), whereas triangles indicate the percentage of apoptotic cells (DAPI staining). (B) NB4 cells (300,000/mL) were treated for 16 hours with the indicated concentration of CD437. Solid circles indicate the percentage of viable cells, whereas open circles indicate the percentage of apoptotic cells. (C and D) Extracts from the cells of the experiments presented in (A) and (B), respectively, were assayed for DEVD-amc hydrolytic activity. Open circles represent extracts from vehicle-treated cells, whereas solid circles represent CD437-treated cells. Results are the mean ± SD of three separate culture dishes.
