KSHV DNA sequences viral cyclin D (ORF 72) and T1.1 (ORF K7) are detectable in MM BMSCs. (A) DNA from long-term BMSCs from 4 patients with MM (lanes 2 through 5) and 2 normal donors (lanes 6 and 7) were assayed by nested PCR to amplify viral cyclin D (ORF 72) KSHV gene sequence. DNA from BCBL-1 cell line (lane 1) served as a positive control and water (H₂O) (lane 8) served as control for nested PCR contamination. (B) DNA from long-term BMSCS from 2 patients with MM (lanes 2 and 3) and 2 normal donors (lanes 5 and 6) were assayed by nested PCR to amplify T1.1 (ORF K7). DNA from BCBL-1 cell line (lane 1) served as a positive control and water (H₂O) (lane 4) served as a control for nested PCR contamination.