Reconstitution of OSM receptor in Ba/F3 cells. (A) Scatchard plot analysis of Ba/F3 transfectant expressing only mOSMRβ; mOSM binds only to mOSMRβ with low affinity. (B) Chemical cross-linking experiment using Ba/F3 transfectant expressing only mOSMRβ; ¹²⁵I-mOSM binds to mOSMRβ specifically. (C) Scatchard plot analysis of Ba/F3 transfectant expressing mOSMRβ and gp130; double transfectant exhibits high- and low-affinity binding sites. (D) Growth stimulation of transfectant expressing mOSMRβ and gp130 responding to various ligands. Double transfectant proliferates in a mOSM-dependent manner, while neither hOSM nor mLIF stimulate its growth.