Fig. 2.
Expression of sst2, sst3, and sst5 mRNA. RNA was extracted and cDNA was synthesized as described in Materials and Methods. Only RNA free of contaminating genomic DNA was used for the PCR. A PCR with 35 cycles of amplification was performed and the products were resolved on a 2% agarose gel and stained with ethidium bromide. dH2O was used as a control for carry-over contamination. The expected sizes of the fragments are: sst2, 342 bp; sst3, 361 bp; sst5, 222 bp.
