Fig. 3.
PNH cells lack proaerolysin binding proteins. Proaerolysin binding to proteins from LD− cells (lane 1), LD−(PIGA+) cells (lane 2), and CEM cells (lane 3). Equal numbers of cells (6 × 104/lane) were applied to each lane. Lane 1 ( LD− cells) is blank, whereas lane 2 [LD−(PIGA+)] has multiple bands. For the sake of comparison, an immature human T-cell line (CEM) was also run in this experiment. A band corresponding to Thy-1, which we have detected previously in mouse T cells, is easily visible in the figure.