(A) Schematic representation of human EPO cDNA, and location of restriction sites DdeI, NcoI, andBglII within the 3′ UTR used to generate EPO transcripts. The hatched area represents the HIPBS-like region. TGA - translation stop codon. Bracket marks the cDNA used to generate the riboprobe. (B) Alignment of pyrimidine-rich tracts in the 3′ UTR of EPO mRNA from different species. Sequences start with the translation stop codon. The first pyrimidine-rich tract conserved in primates is indicated by italics and underlined. The second motif conserved in various species is indicated by bold type and underlined. Gene bank accession numbers for each EPO mRNA are X02157 (human), M18189(monkey), L10608 (rat), M12482 (mouse), L10607 (swine), U44762(bovine). (C) Sequences of wild-type TH and EPO HIPBS elements, and localization of mutations (MUT). The G residue within the protein binding site is underlined.