Formation of the ribonucleoprotein complex associated with EPO mRNA is not hypoxia-inducible. (A) Gel-shift analysis of the protein complexes associated with EPOBglII (lanes 1 to 5), EPO DdeI (lanes 6 to 10) transcripts or EPO HIPBS (lanes 11 to 15) and protein extracts (40 μg) from either Hep3B or HepG2 cells during normoxia (21% O2) and hypoxia (1% O2). (B) SDS-PAGE analysis of UV light cross-linked complexes formed by EPO DdeI-transcript and protein factors (40 μg) from Hep3B (lanes 1, 2), HepG2 (lanes 3, 4), and PC12 cells (lanes 5, 6) that were exposed to normoxia (21% O2) or hypoxia (1% O2). (C) SDS-PAGE analysis of UV light cross-linked complexes formed by TH transcript and protein factors from PC12 (10 μg, lanes 2, 3), Hep3B (40 μg, lanes 4, 5), and HepG2 cells (40 μg, lanes 6, 7) that were exposed to normoxia (21% O2) or hypoxia (1% O2). FP - free probe.
