Fig. 1.
Examples of the analysis of cells from 2 patients showing the DAPI, SKY, and classified images as well as the results using painting probes. (Top two rows, Case 1) (Left) Reverse DAPI image with the der(1), der(5), and der (6) chromosomes as well as normal 9 and del (9p) identified by arrows. (Middle) SKY image; (right) classified image. (Second row right) The classified karyotype of this cell. (Second row) Three panels show the results with painting probes for chromosomes 1, 5, and 6, confirming (left) that chromosome 5 material is on both the der(1) and der(6), (middle left) that the t(1;5) is reciprocal, and (middle right) that there is no material from chromosome 6 on either the der(1) or the der(5). (Bottom two rows, Case 3) (Left) Reverse DAPI image with the abnormal chromosomes including two Y chromosomes and dup(1p), del(7p), der(14), der(18), and der(19), are identified by arrows. (Middle) SKY image of same cell; (right) classified image. (Bottom row right) Classified karyotype. (Bottom row) Three panels showing the results of painting probes for chromosomes 4, 7, and 9, confirming (left) that part of chromosome 4 is on the der(18), (middle left) that part of 7, presumably 7p, is on the der(14), and (middle right) that part of 9 is on the der(19).