Fig. 5.
![Fig. 5. PI-6 coimmunoprecipitates with cathepsin G from U937 cells as a 56-kD complex. (A) U937 cells were [35S]methionine labeled, lysed, and immunoprecipitated with rabbit polyclonal antibodies recognizing cathepsin G, proteinase 3, or human leukocyte elastase. Immune complexes were separated by 12.5% SDS-PAGE under reducing conditions. (B) U937 cells were lysed and incubated at 37°C for 10 minutes. Extracts were immunoprecipitated with rabbit polyclonal antibodies recognizing cathepsin G, proteinase 3, or human leukocyte elastase. Immune complexes were separated by 12.5% SDS-PAGE and separated proteins were immunoblotted using monoclonal 3A anti–PI-6 antibody.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/93/6/10.1182_blood.v93.6.2089.406k10_2089_2097/5/blod40610005bw.jpeg?Expires=1735508206&Signature=LC-3PUYdZ4nUlG0eGv7vnrFGXQRvO~tpmNOeeuzX-KlYtcsRXdvvgSoMrBbV-Z1aPDpSy5eEOpx3srD7N8Q3zllPkglobHYAdVMNOSUoi8k~df47ozQ-XaPmcmKmIX-KmWMkT6meVwLiejzPzMPs7A5toXaVr6caL5-OEq2DSU17ZZfpSfVtj6I7qFKgySVxEYG25bOsni64ifDi0HZHscpQbmJus3wxABOIIhSJ8Bwf-8ddNEbnexmmgWU9mba3bzFgR~Mc6bMSroTFiMhIZUZ3aRADuA7rs3qPtNRMW-a4Ajk7xObQZ1g~Ott~ARHSfBvwnP8m7ko5BwMBQPY0oQ__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
PI-6 coimmunoprecipitates with cathepsin G from U937 cells as a 56-kD complex. (A) U937 cells were [35S]methionine labeled, lysed, and immunoprecipitated with rabbit polyclonal antibodies recognizing cathepsin G, proteinase 3, or human leukocyte elastase. Immune complexes were separated by 12.5% SDS-PAGE under reducing conditions. (B) U937 cells were lysed and incubated at 37°C for 10 minutes. Extracts were immunoprecipitated with rabbit polyclonal antibodies recognizing cathepsin G, proteinase 3, or human leukocyte elastase. Immune complexes were separated by 12.5% SDS-PAGE and separated proteins were immunoblotted using monoclonal 3A anti–PI-6 antibody.
