Fig. 2.
Fig. 2. Examination of cell cycle activity from BM and spleen from FR25 Lin− and R/O cells pre- and posttransplant (48 hours). A, B, and C represent FR25Lin−propidium iodide (PI)-labeled cell cycle analyses of preinjection (A), 48 hours in the BM (B), and 48 hours in the spleen (C). D, E, and F are R/O PI cell cycle analyses of preinjection (D), 48 hours in the BM (E), and 48 hours in the spleen (F). Values represent the mean ± standard error of mean (SEM) for five experiments. Comparison of A to B and A to C show no statistically significant differences for S phase. Statistical analysis showed significant difference when comparing D to E (P < .03) and D to F (P < .01) for the S phase of the cell cycle. The histograms and the curve fitting software allowed the determination of the percentage of cells in the phases of the cell cycle (Multi-cycle from Phoenix Flow Systems Inc, San Diego, CA).

Examination of cell cycle activity from BM and spleen from FR25 Lin− and R/O cells pre- and posttransplant (48 hours). A, B, and C represent FR25Lin−propidium iodide (PI)-labeled cell cycle analyses of preinjection (A), 48 hours in the BM (B), and 48 hours in the spleen (C). D, E, and F are R/O PI cell cycle analyses of preinjection (D), 48 hours in the BM (E), and 48 hours in the spleen (F). Values represent the mean ± standard error of mean (SEM) for five experiments. Comparison of A to B and A to C show no statistically significant differences for S phase. Statistical analysis showed significant difference when comparing D to E (P < .03) and D to F (P < .01) for the S phase of the cell cycle. The histograms and the curve fitting software allowed the determination of the percentage of cells in the phases of the cell cycle (Multi-cycle from Phoenix Flow Systems Inc, San Diego, CA).

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