H-Ras expression in nontransfected Baf3 cells (designated as Baf3/c) and wild-type, constitutively active type, and dominant-negative type H-Ras–transfected Baf3 cells (designated as wild-type, constitutively active, and dominant-negative, respectively). Cells were lysed in lysis buffer, separated by 15% SDS-PAGE, immunoblotted with anti–H-Ras antibody, and then stripped and reblotted with anti-GAPDH antibody (as an internal control). This is a representative result of three independent experiments.