Effects of inhibitor compounds on constitutively active type H-Ras–transfected Baf3 cells (designated as constitutively active) adhesion to FN without IL-3 stimulation and constitutively active type H-Ras–transfected Baf3 cells, wild-type H-Ras–transfected Baf3 cells (designated as wild-type), and dominant-negative type H-Ras–transfected Baf3 cells (designated as dominant-negative) adhesion to FN induced by IL-3. Those transfected Baf3 cells were labeled with 51Cr and incubated at 37°C for 30 minutes in the absence or presence of 100 nmol/L wortmannin, 1 μmol/L U-73122, 1 μmol/L U-73433, or 50 μmol/L H-7. Cells were subsequently transferred to FN-coated wells and incubated for an additional 30 minutes at 37°C with or without 0.1 ng/mL IL-3, and the percentage of adherent cells was measured. Data represent the means (±SD) of triplicate samples from a representative experiment of three. None of the inhibitor compounds had an effect on viability of the cells. *P value (P < .01) comparing adhesion blocked by U-73122 with control adhesion.
