Fig. 7.
Induction of the adhesion to FN of nontransfected Baf3 cells (designated as Baf3/c), wild-type H-Ras–transfected Baf3 cells (designated as wild-type), constitutively active type H-Ras–transfected Baf3 cells (designated as constitutively active), and dominant-negative type H-Ras–transfected Baf3 cells (designated as dominant-negative) by anti-β1 integrin antibody (clone; 9EG7). These Baf3 cells were labeled with 51Cr and incubated at 37°C for 30 minutes in the presence of 5 μg/mL of anti-β1 integrin antibody (clone; 9EG7) or isotype-matched control antibody. Cells were subsequently transferred to FN-coated wells and incubated at 37°C for an additional 30 minutes, and the percentage of adherent cells was measured. Data represent the means (±SD) of triplicate samples from a representative experiment of three. *P value (P < .01) comparing adhesion induced by anti-β1 integrin antibody with the control antibody.