American Society of Hematology

Fig. 6.

$Fig. 6. Purification of GST-Vpr and Vpr. The HIV-1 (strain NL4-3) Vpr coding sequence was cloned into the GST fusion protein bacterial expression vector, pGEX. The GST-Vpr fusion protein, synthesized after induction of the bacterial cells with 0.1 mmol/L IPTG, was purified (lane 1) by binding to glutathione agarose. Free Vpr was obtained by proteolytic digestion of the GST-Vpr protein and collecting the flow-through fraction from glutathione resin. The purity was assessed by Western blotting analysis using a rabbit polyclonal antisera against Vpr.$

Purification of GST-Vpr and Vpr. The HIV-1 (strain NL4-3) Vpr coding sequence was cloned into the GST fusion protein bacterial expression vector, pGEX. The GST-Vpr fusion protein, synthesized after induction of the bacterial cells with 0.1 mmol/L IPTG, was purified (lane 1) by binding to glutathione agarose. Free Vpr was obtained by proteolytic digestion of the GST-Vpr protein and collecting the flow-through fraction from glutathione resin. The purity was assessed by Western blotting analysis using a rabbit polyclonal antisera against Vpr.

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