American Society of Hematology

Fig. 1.

$Fig. 1. (A) Induction of IL-2R, c-myc, andpim-1 gene expression by IFN-, IL-2, IL-12, and IL-15 in human T lymphocytes. T cells were stimulated with different cytokines for 3 hours, the cells were collected, and the total cellular RNA was isolated. RNA samples (20 μg) were size-fractionated on agarose gels, transferred to nylon membranes, and hybridized with specificIL-2R, c-myc, and pim-1 cDNA probes. EtBr-stained gel is shown to verify equal RNA loading. The result shown is from one experiment but is representative of three individual experiments. (B) Induction of IL-2R protein expression by IFN-, IL-2, IL-12, and IL-15. T cells were stimulated with different cytokines for 24 hours, and cell lysates were prepared. Proteins were separated on 10% SDS-PAGE, transferred to membranes, and immunoblotted with anti–IL-2R antibody.$

(A) Induction of IL-2R, c-myc, andpim-1 gene expression by IFN-, IL-2, IL-12, and IL-15 in human T lymphocytes. T cells were stimulated with different cytokines for 3 hours, the cells were collected, and the total cellular RNA was isolated. RNA samples (20 μg) were size-fractionated on agarose gels, transferred to nylon membranes, and hybridized with specificIL-2R, c-myc, and pim-1 cDNA probes. EtBr-stained gel is shown to verify equal RNA loading. The result shown is from one experiment but is representative of three individual experiments. (B) Induction of IL-2R protein expression by IFN-, IL-2, IL-12, and IL-15. T cells were stimulated with different cytokines for 24 hours, and cell lysates were prepared. Proteins were separated on 10% SDS-PAGE, transferred to membranes, and immunoblotted with anti–IL-2R antibody.

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