American Society of Hematology

Fig. 2.

$Fig. 2. Dose-dependent activation of IL-2R, c-myc, andpim-1 gene expression by type I IFNs. T cells were stimulated with different doses of IFN- or IFN-β for 3 hours, the cells were collected, and total cellular RNA was isolated. RNA samples (20 μg) were size-fractionated on agarose gels, transferred to nylon membranes, and hybridized with IL-2R, c-myc, and pim-1 cDNA probes. EtBr-stained gel is shown to verify equal RNA loading.$

Dose-dependent activation of IL-2R, c-myc, andpim-1 gene expression by type I IFNs. T cells were stimulated with different doses of IFN- or IFN-β for 3 hours, the cells were collected, and total cellular RNA was isolated. RNA samples (20 μg) were size-fractionated on agarose gels, transferred to nylon membranes, and hybridized with IL-2R, c-myc, and pim-1 cDNA probes. EtBr-stained gel is shown to verify equal RNA loading.

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