Carotid artery blood flow tracings obtained from a wild-type mouse and a VN-deficient mouse. Period of ferric chloride injury is indicated by hatched bar. Artifactual reduction in flow during injury is due to removal of saline from the surgical site to allow application of FeCl₃. Thrombotic occlusion occurs 18.7 minutes and ≤3.0 minutes after initiation of injury in theVN +/+ mouse and theVN −/− mouse, respectively.