Maps of AAV vectors used. Bold arrows denote the direction of transcription of each gene. The promoters, polyadenylation signals (PA), and the AAV ITR are shown. Light arrows denote the location of PCR primers. Primers RPSP and DLASP were used for amplification from DNA templates. Primers 1a and 1b were used for reverse transcription and amplification from RNA templates. Probes used for FISH analyses and Southern hybridization of RT-PCRs and dot blot analyses of vectors are designated. (A) vCWRHIVASVN encodes two transgenes: (1) an antisense RNA (A) complementary to the HIV LTR, including the TAR and the polyadenylation sequences under the transcriptional control of the RSV LTR; and (2) the neomycin phosphotransferase (Neo^R) gene under the control of the SV40 early promoter. (B) vCWRHIVAPAP contains the antisense RNA gene cassette described in (B) in addition to the PLAP gene under the control of the PGK promoter.