Induction of alloantigen-specific anergy in CD8⁺ T cells by IL-10–treated DC. Purified naive CD8⁺ T cells were cultured in medium alone; with untreated, control DC; or with DC pretreated with IL-10 (first coculture). After 36 hours, T cells were rescued, cultured for 1 to 7 days in medium containing low levels of IL-2 (2 U/mL), and subsequently restimulated (second coculture) with untreated, mature DC generated from the same donor (░) or a second unrelated donor (▪). [³H] TdR incorporation was determinated after 48 hours. The left bars show the antigen-specific response and the right bars show the T-cell response to IL-2 (100 U/mL).