Induction of melanoma antigen-specific anergy in tyrosinase-specific, cytotoxic CD8⁺ T cells. In a first coculture, tyrosinase-specific HLA.A2⁺ CD8⁺T cells were used as responder cells for HLA.A2⁺ DC additionally pretreated with IL-10 or untreated (cultured in complete medium alone). Cultures were set up in the presence of the specific tyrosinase peptide or an unspecific control peptide (MART-1). Subsequently, the T cells were rescued, cultured for 1 to 7 days in the presence of low levels of IL-2 (2 U/mL), and restimulated with mature HLA.A2⁺ DC during the second coculture. After 2 days, the proliferation was measured by [³H]TdR incorporation. The left bars show the specific response and the right bars show the T-cell response to IL-2 (100 U/mL). The results are representative for three experiments.