Anergic tyrosinase-specific CTL fail to lyse tumor cells. Control (precultured with untreated DC) and anergic (precultured with IL-10–treated DC) tyrosinase-specific HLA.A2⁺CD8⁺ T cells were cocultured with the HLA.A2⁺ tyrosinase-expressing melanoma cell line SK-MEL 28 in a ⁵¹Cr-release assay for 4 hours. Experiments with specific CD8⁺ T cells cocultured with MART-1 during the primary culture served as controls. Various effector:T-cell ratios were used in the experimental setting. The percentage of specific lysis was calculated from the average of triplicates as 100 × (⁵¹Cr-release into supernatant − spontaneous release)/(total release in detergent − spontaneous release). All synthetic peptides were tested for nonspecific lysis of target cells in the absence of CTL.