Restriction analysis for confirmation of the exon 5 mutation, analysis of mutation origin, and genotype analysis for the C282Y HFE mutation in family 5. (A) Sau3AI restriction of exon 5 PCR products. (B) Polymorphic allele haplotype for the CA repeat in intron 7 of the ALAS2 gene. (C) RsaI restriction analysis of PCR products encompassing the C282 codon of theHFE gene.