The development of hematopoietic progenitor cells in cultured AGM cells. (A) Primary and cultured AGM cells were analyzed with TEK, CD34, and c-Kit MoAb by FACS. Embryo 10.5 dpc AGM cells were cultured on OP9 cells and they developed Sca-1⁺ and CD34⁺ cells in 14 days of culture. (B) Lin⁻, c-Kit⁺, Sca-1⁺ cells consisted of 3.2% of the cultured AGM cell population.