Altered differentiation of LN-ras2–transduced BM cells. (A through D) FACS analysis of transduced BM cells after 27 days in liquid culture. (A) LK cells stained with Mac-1 antibody (macrophage specific). (B) LK cells stained with Gr-1 (granulocyte specific). Underlayed (open curves represent isotype controls). (C) LN-ras2 cells stained with Mac-1. (D) LN-ras2 cells stained with Gr-1. (E and F) Cytocentrifuge preparations stained with May-Grunwald and Giemsa showing morphologic appearance of methylcellulose colonies after 7 days in liquid culture. View shows representative cells from pooled colonies from LK (E) and LN-ras2 (F) cultures (original magnification ×500).