Fig. 1.
Fig. 1. MDR in MPC11Dox10-1 cells is conferred bymdr1/Pgp. (A) Detection of increased mdr1 mRNA in MPC11Dox10-1 cells by RT-PCR. Total RNA was isolated from MPC11 and MPC11Dox10-1 cells, reverse transcribed, and amplified with primers specific for the mdr1 and β-actin genes as described in Materials and Methods. The experiments were repeated twice with the same results. (B) Calcein accumulation is decreased in MPC11Dox10-1 cells. MPC11 (upper panel, profiles 1 through 3) and MPC11Dox10-1 (lower panel, profiles 4 through 6) cells were left untreated (1 and 4) or treated with 50 nmol/L calcein/AM in the absence (2 and 5) or presence (3 and 6) of 40 μmol/L verapamil. After washing, cellular fluorescence was measured on a FACScan (excitation 488 nm, emission 524 nm) on FL1. The experiments were performed twice with comparable results.

MDR in MPC11Dox10-1 cells is conferred bymdr1/Pgp. (A) Detection of increased mdr1 mRNA in MPC11Dox10-1 cells by RT-PCR. Total RNA was isolated from MPC11 and MPC11Dox10-1 cells, reverse transcribed, and amplified with primers specific for the mdr1 and β-actin genes as described in Materials and Methods. The experiments were repeated twice with the same results. (B) Calcein accumulation is decreased in MPC11Dox10-1 cells. MPC11 (upper panel, profiles 1 through 3) and MPC11Dox10-1 (lower panel, profiles 4 through 6) cells were left untreated (1 and 4) or treated with 50 nmol/L calcein/AM in the absence (2 and 5) or presence (3 and 6) of 40 μmol/L verapamil. After washing, cellular fluorescence was measured on a FACScan (excitation 488 nm, emission 524 nm) on FL1. The experiments were performed twice with comparable results.

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