SHIP expression in BM and BMM. (A) Murine BM was isolated and treated as described in Materials and Methods. Total RNA was purified from undifferentiated BM or from BMM. RT-PCR was performed using the primer sets shown in Fig 1A. (B) Untreated murine BMCs or BMM were lysed in NP-40 lysis buffer. Equal amounts (40 μg) of total protein were loaded into each lane of a 6% polyacrylamide gel. The gels were processed as described and protein was detected with the anti-SHIP polyclonal antibody #5340.