Human SHIP 135 kD protein. (A) ML-1 human monocytic leukemia cells were treated for 2 days with TPA, and adherent cells were lysed and subjected to SDS-PAGE. Blots were incubated with SHIP polyclonal antibodies #5340 or #5369. (B) RNA from ML-1 cells with the same TPA treatment was analyzed by RT-PCR using primers to human SHIP 2615-3231 (Genbank accession number U84400). Two bands were detected at approximately 600 and 350 bp. These were purified and subjected to DNA sequencing, and the results are depicted as in Fig 2A.