Fig. 5.
Proliferative responses of purified allogeneic CD3+ T cells in the absence of IL-2 to γ-irradiated B-CLL cells (patient no. 12) either unstimulated or stimulated for 24, 72, and 120 hours by CD40L-transfected NIH3T3 fibroblasts. [3H] Thymidine incorporation was assessed for the last 12 hours of a 3-day culture. Appropriate controls (CD3+ T cells and B-CLL cells) were always less than 1,500 cpm. The stimulation index was calculated as cpm(T cells + B-CLL cells)/cpm(T cells). Results are representative for three independent experiments and are expressed as the mean ± SD of the stimulation index.