Surface phenotype before and after the induction into DCs. Cells were grown for 3 weeks in FLT3-L plus TPO, frozen, and thawed after 3 months of cryopreservation. Cells were then cultured back in the initial conditions for 10 days (left column). Cells were induced with GM-CSF plus IL-4 for 2 days (middle column) or with GM-CSF and IL-4 for 2 days, followed by 3 more days with the latter cytokines plus TNF (right column). One representative experiment is shown out of four.