Representative photographs of May-Grünwald-Giemsa–stained cytospin preparations of cells cultured with different cocktails of cytokines after primary cultures. (A) Unsorted cells in GM-CSF, IL-4, and TNF for 7 days, (B) p14⁺ cells 3 days in GM-CSF plus IL-4. (C) p14⁺ cells, 7 days with the same cytokines as (B), with adjunction of TNF for the last 4 days. (D and E) p14⁻cells after 9 days of culture with GM-CSF plus TNF or GM-CSF plus FLT3-L, respectively. (F) p14⁻ cells 3 days with GM-CSF plus FLT3-L, 4 days with GM-CSF and IL-4, then 4 days with GM-CSF plus IL-4 and TNF. (A, B, and C) Original magnification ×400. (D, E, and F) Original magnification ×250.