Fig. 3.
Fig. 3. Expression of CD11b and CD14 antigens by FACS analysis. UF-1 cells were treated with either 10−7 mol/L all-trans RA, various concentrations (10−9 to 10−7 mol/L) of 1,25(OH)2D3, or combinations of both for 4 days. Cells were incubated for 30 minutes with human AB serum to block Fc receptors and then stained with direct immunofluorescence using FITC-conjugated mouse anti–human CD14 and PE-conjugated mouse anti–human CD11b antibodies. Control studies were performed with nonbinding control mouse IgG1 and IgG2a isotype antibodies.

Expression of CD11b and CD14 antigens by FACS analysis. UF-1 cells were treated with either 10−7 mol/L all-trans RA, various concentrations (10−9 to 10−7 mol/L) of 1,25(OH)2D3, or combinations of both for 4 days. Cells were incubated for 30 minutes with human AB serum to block Fc receptors and then stained with direct immunofluorescence using FITC-conjugated mouse anti–human CD14 and PE-conjugated mouse anti–human CD11b antibodies. Control studies were performed with nonbinding control mouse IgG1 and IgG2a isotype antibodies.

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