Fig. 5.
![Fig. 5. Expression of p21WAF1/CIP1 mRNA in HL-60 and NB4 cells, and p21WAF1/CIP1 and p27KIP1 transcripts in UF-1 cells. Cells were cultured with either 10−7 mol/L 1,25(OH)2D3 (A) or 10−7 mol/L all-trans RA (B) for 48 hours. Total RNA (15 μg per lane) was extracted and analyzed by Northern blot with [32P]-labeled p21WAF1/CIP1 or p27KIP1 cDNA probe. Bottom panel shows the ethidium bromide–stained formaldehyde gel before Northern blotting; the levels of 28S and 18S ribosomal RNA are comparable in each lane.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/93/7/10.1182_blood.v93.7.2225/5/blod40705005w.jpeg?Expires=1735558236&Signature=T1UvjFiTN7K9nWl9mTFrOJRQTPRoAflfMO4xn-z3v5TeFWrhV963KxlPbVIcWUMMmg9PlLJAP0OVss5liB0PIl4vWCUhBHc7CZPo5P1-afm2NoJ3gqeeqG62anlCp4Rq58cyV6-59YWpyb~7aJgkOL~W1fJVpDSloCYAbl~pq9169oDWvWQ6Ew3ktFW87jNE9NLkmdUdKuV9aleXxqJfpae3ODFCWO3Qgkd2N2l978999Vg9YPJcWfiTrERdtmbtovSs77g7ydnbpO7X1XL-uI7DECGIgLszULuxrCYHvDw2mien~5gt2mXkxc6-Z0Ni4FxNFaQiABev~V6n3AbSuA__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Expression of p21WAF1/CIP1 mRNA in HL-60 and NB4 cells, and p21WAF1/CIP1 and p27KIP1 transcripts in UF-1 cells. Cells were cultured with either 10−7 mol/L 1,25(OH)2D3 (A) or 10−7 mol/L all-trans RA (B) for 48 hours. Total RNA (15 μg per lane) was extracted and analyzed by Northern blot with [32P]-labeled p21WAF1/CIP1 or p27KIP1 cDNA probe. Bottom panel shows the ethidium bromide–stained formaldehyde gel before Northern blotting; the levels of 28S and 18S ribosomal RNA are comparable in each lane.
