Fig. 8.
Fig. 8. Western blot analysis of p21WAF1/CIP1and p27KIP1 protein. (A) Total cellular protein (10 μg per lane) from UF-1 cells treated for 3-72 hours with 10−7 mol/L 1,25(OH)2D3 was separated on a 12.5% SDS-polyacrylamide gel and transferred to the membrane. p21WAF1/CIP1 and p27KIP1 protein levels were detected by Western blotting using antibodies directed against p21WAF1/CIP1 and p27KIP1, and then the blots were stained with Coomassie brilliant blue to confirm that equal amounts of protein were present in each lane. (B) Enhanced expression of p27KIP1 protein by exposure of cells to the combination of 10−7 mol/L all-trans RA and 1,25(OH)2D3 for 48 hours.

Western blot analysis of p21WAF1/CIP1and p27KIP1 protein. (A) Total cellular protein (10 μg per lane) from UF-1 cells treated for 3-72 hours with 10−7 mol/L 1,25(OH)2D3 was separated on a 12.5% SDS-polyacrylamide gel and transferred to the membrane. p21WAF1/CIP1 and p27KIP1 protein levels were detected by Western blotting using antibodies directed against p21WAF1/CIP1 and p27KIP1, and then the blots were stained with Coomassie brilliant blue to confirm that equal amounts of protein were present in each lane. (B) Enhanced expression of p27KIP1 protein by exposure of cells to the combination of 10−7 mol/L all-trans RA and 1,25(OH)2D3 for 48 hours.

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