American Society of Hematology

Fig. 9.

$Fig. 9. Flow cytometric analysis for markers of cellular differentiation on p27KIP1-transfected HL-60 cells. Cells were transiently transfected with either pEGFP-hKIP1 or pEGFP-neo control plasmid by electroporation. Transfected cells were cultured for 48 hours and then stained with PE-conjugated mouse anti–human CD11b antibody. Expression of CD11b antigen in the GFP-positive fraction was analyzed by two-color flow cytometry.$

Flow cytometric analysis for markers of cellular differentiation on p27^KIP1-transfected HL-60 cells. Cells were transiently transfected with either pEGFP-hKIP1 or pEGFP-neo control plasmid by electroporation. Transfected cells were cultured for 48 hours and then stained with PE-conjugated mouse anti–human CD11b antibody. Expression of CD11b antigen in the GFP-positive fraction was analyzed by two-color flow cytometry.

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