Competition between LE antibodies and MoAbESH8 for binding on rFVIII. Microtiter plates were coated with 5 μg/mL MoAbESH8 before addition of biotinylated rFVIII (1 μg/mL) alone (▪) or complexed to vWF (□) for an incubation of 2 hours at RT. Incubations were made in the presence of either LE IgG (1,000 μg/mL), IgG from a normal donor (Nl) (1,000 μg/mL), or MoAbESH8 (5 μg/mL), or in the absence of antibody (buffer). The mean of triplicates with SD are indicated.