Fig. 1.
Growth of multipotential hematopoietic cell lines on stromal cells is suppressed by ligation of CD9. EML-C1 cells (A) or Myl-D-7 cells (B) were added to 12-well plates (1 × 105cells/well) precoated with MS-5 cells and cocultured for 7 days in media containing 1 μg/mL KMC8.8 or isotype-matched antibody. 10 ng/mL SCF was also present in the EML-C1 cell cocultures. The cultures were harvested by treatment with EDTA plus trypsin and numbers of recovered cells were determined with a hemocytometer. Results represent means ± SD of triplicate cultures. Statistically significant differences from control values are indicated by one (P < .05) or two (P < .01) asterisks.
