The clonogenic capacity of EML-C1 cells is altered after coculture with MS-5 stromal cells in the presence of a CD9 antibody. EML-C1 cells (1 × 104) were placed in 12-well plates precoated with MS-5 and cocultured for 7 days in the presence of 1 μg/mL KMC8.8 or an isotype-matched control antibody. After removal from stromal cells and washing, recovered EML-C1 cells were counted and placed in methylcellulose assays with different combinations of growth factors. CFU-Blast colonies (A) were detected with 50 ng/mL SCF, CFU-GM (B) were elicited with 50 ng/mL SCF + 2 ng/mL IL-3, and BFU-E (C) were cultivated with 50 ng/mL SCF + 8 U/mL EPO. Results represent the mean ± SD of triplicate cultures. Statistically significant differences from control values are indicated by one ( P < .05) asterisk.
