Antigen ligation of CD9 on MS-5 stromal cells augments adhesion of EML-C1 stem cell line. (A) EML-C1 cells (5 × 105) were plated on MS-5 cells in the presence of the indicated concentrations of KMC8.8 for 30 minutes. Adhesion of EML-C1 cells to MS-5 cells was evaluated by cell adhesion assays. (B) EML-C1 cells (5 × 105) were plated on MS-5 cells in the presence of 1 μg/mL KMC8.8 for the indicated periods of time, and then cell adhesion assays were performed. (C) F(ab′)2 fragments of KMC8.8 were prepared using ImmunoPure F(ab′)2Preparation Kit. EML-C1 cells (5 × 105) were plated on MS-5 cells in the presence of 1 μ g/mL of F(ab′)2fragments of KMC8.8, intact KMC8.8, or a control antibody for 30 minutes, and then cell adhesion assays were performed. (D) EML-C1 cells or MS-5 cells were preincubated with 1 μg/mL of the indicated antibodies for 30 minutes, and washed three times. Subsequently, EML-C1 cells (5 × 105) were plated on MS-5 cells, and then cell adhesion assays were performed. Results represent the mean ± SD of triplicate cultures. Statistically significant differences from control values are indicated by one (P < .05) or two (P < .01) asterisks.
