Fig. 1.
Fig. 1. Homologous recombination-mediated disruption of the 7.2b gene in mice. (A) Schematic representation of the 5′ region of the 7.2b gene including exon 1, intron 1, exon 2, and part of intron 2. B, BamHI sites. Shaded rectangles are the regions included in the targeting vector. (B) The 21-kb targeting vector. Neo, bacterial neomycin resistance gene; tk, herpes simplex virus thymidine kinase gene cassette; GHt, human growth hormone gene-N polyA-signal/terminator. (C) Structure of targeting event. 7.2KO-3 and 7.2KO-4, PCR primers used to screen for targeting event; probe A, probe used for genotyping by Southern analysis. (D) Genotyping mice by Southern blot analysis. BamHI cut genomic DNA probed with probe A. +/+, wild-type mice; +/−, mice heterozygous for 7.2b targeting event; −/−, mice homozygous for 7.2b targeting event. Size markers (kb) are from a 1-kb DNA ladder (GIBCO-BRL, Gaithersburg, MD).

Homologous recombination-mediated disruption of the 7.2b gene in mice. (A) Schematic representation of the 5′ region of the 7.2b gene including exon 1, intron 1, exon 2, and part of intron 2. B, BamHI sites. Shaded rectangles are the regions included in the targeting vector. (B) The 21-kb targeting vector. Neo, bacterial neomycin resistance gene; tk, herpes simplex virus thymidine kinase gene cassette; GHt, human growth hormone gene-N polyA-signal/terminator. (C) Structure of targeting event. 7.2KO-3 and 7.2KO-4, PCR primers used to screen for targeting event; probe A, probe used for genotyping by Southern analysis. (D) Genotyping mice by Southern blot analysis. BamHI cut genomic DNA probed with probe A. +/+, wild-type mice; +/−, mice heterozygous for 7.2b targeting event; −/−, mice homozygous for 7.2b targeting event. Size markers (kb) are from a 1-kb DNA ladder (GIBCO-BRL, Gaithersburg, MD).

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