Fig. 2.
![Fig. 2. Constitutive activation of a STAT-like DNA-binding factor in Dami/HEL and Meg-01 cells. Nuclear extracts from each cell line cultured in the absence of cytokine (CTL) or in the presence of as much as 400 ng/mL TPO or 40 ng/mL IL-3, were incubated with [32P]-labeled, double-stranded IRF-1 GAS oligonucleotide. The DNA-binding complex and unbound probes were separated electrophoretically on 5% nondenaturing polyacrylamide gels. The autoradiograph shows the STAT-like DNA-binding factor (DBF) and the nonspecific bands (NS).](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/93/7/10.1182_blood.v93.7.2369/5/blod40718002w.jpeg?Expires=1735505548&Signature=pKLu7v8LorBVUBzJk-dS4vdcf4RenGtgy0mF9MASa8Qd1ECIpQp1IkC93Nk5B8yCBrTQAssV6JcDX3X1gn6S~mSibArmxU6iUsB9k-OTlYn6DE4n1m90UTH-WfJ2~Xo477GCDb1U58buF2eZL~a5h1yRd7Wq5coj0qzJYSuhjXXAwQNaD09z6S65ZkwMWHst9dO~jSGHYYoBSjEiA5dHRopynoknAo64P85HnsdO9GIFb~3EloQgiETQhJipIJ2xqnC82~qwkkuz2x6q~bT4vS-w75a0d4yxHkj7~sBn4moxuCTSLGHw8ULkLspinWz8iQzz9ypZnV0zMm3-QMkHzA__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Constitutive activation of a STAT-like DNA-binding factor in Dami/HEL and Meg-01 cells. Nuclear extracts from each cell line cultured in the absence of cytokine (CTL) or in the presence of as much as 400 ng/mL TPO or 40 ng/mL IL-3, were incubated with [32P]-labeled, double-stranded IRF-1 GAS oligonucleotide. The DNA-binding complex and unbound probes were separated electrophoretically on 5% nondenaturing polyacrylamide gels. The autoradiograph shows the STAT-like DNA-binding factor (DBF) and the nonspecific bands (NS).
