Fig. 3.
![Fig. 3. Effects of cytokines on activation of a STAT-like DNA-binding factor in Mo7e cells. Nuclear extracts from Mo7e cells grown in the absence of cytokine (CTL), or in the presence of 40 ng/mL EPO, 400 ng/mL TPO, 40 ng/mL GM-CSF or 40 ng/mL IL-3, were incubated with [32P]-labeled IRF-1 GAS oligonucleotide. The STAT-like DNA-binding complex and unbound probe were separated on 5% native polyacrylamide gel. The autoradiograph shows the DBF, NS, and free probes (P).](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/93/7/10.1182_blood.v93.7.2369/5/blod40718003w.jpeg?Expires=1735824333&Signature=zWNCVIZCqcYqxH3u5IPlMoT5nk0Rzpaxz6RLSKDlMTToQ67v9WYmX-nVYBG5B27LJC-lN8n8qbDXS-pamZeUeKoPddn1Uejg-M297V6Ivsgh68aFtckV4fs0p2cuZTiLO-~d~YEBaYHUmpW9315fVvXCW~kwwQGaIVGhXr~80ANvufmeZePcEdCCJUKVzyPBNW7q7CPAD4XNEW-VnuohNnECKhVry5yOu3li3YX9nSWH7h12Sz32yl5sXAHZpzx~d2kTCbA-bYoO-3xxOamR~ioEUrmSOuLyodlvRg23GdaDIgWDeKXCG3mkzmw1JNRc-O725tIrn51u3IT76I31ZA__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Effects of cytokines on activation of a STAT-like DNA-binding factor in Mo7e cells. Nuclear extracts from Mo7e cells grown in the absence of cytokine (CTL), or in the presence of 40 ng/mL EPO, 400 ng/mL TPO, 40 ng/mL GM-CSF or 40 ng/mL IL-3, were incubated with [32P]-labeled IRF-1 GAS oligonucleotide. The STAT-like DNA-binding complex and unbound probe were separated on 5% native polyacrylamide gel. The autoradiograph shows the DBF, NS, and free probes (P).
