Fig. 2.
Effect of rHSP-D on IAV-induced neutrophil apoptosis. Various amounts of rHSP-D were preincubated alone or with 25 μg/mL of Bangkok 79 IAV for 30 minutes at 37°C before incubation (for 20 minutes at 37°C) with neutrophils (5 × 106/mL). At indicated times the samples were assessed for apoptosis using the PI flow cytometry assay. Data are expressed as percentage of cells with hypodyploid nuclei and represent mean ± SEM of at least four separate experiments. As indicated by the (*), Bangkok 79 IAV alone caused significant (P < .05) apoptosis. Samples treated with IAV and rHSP-D together did not cause significantly greater apoptosis than did IAV alone. rHSP-D alone did not cause significant apoptosis.
