Effect of IAV, E coli or the combination of IAV and E coli on a percentage of lysed neutrophils and a percentage of control viable neutrophils. These data were obtained on neutrophils treated with 25 μg/mL of IAV, 250 μg/mL of E coli, or both in combination as described in Fig 4. The top panel shows the percent neutrophil viability as determined by the percent of cells able to exclude Trypan blue. In the bottom panel, counts of absolute numbers of viable neutrophils were made (ie, number of cells able to exclude Trypan blue) and divided by number of viable neutrophils present in the control samples (×100 to give percent of control). (*)Indicates samples showing a significant reduction in percent viability as compared with control. (**)Indicates sample with a significant reduction in viable numbers of neutrophils as compared with control samples or samples treated with IAV or E coli alone.