Effect of NO on the activity of OXPHOS complexes (A and B) and cytochrome c release (C) in Jurkat cells transfected only with neomycin (J-Neo) and in Bcl-2 overexpessing Jurkat cells (J-Bcl-2). Evaluation of OXPHOS complex acitivity and cytochromec release was performed as described in the legend to Fig 1. (A) Decrease of OXPHOS complex activity under NO treatment. Data were expressed as a percentage to the untreated controls. (B) NO-mediated suppression of complex IV activity. Data were expressed in fmol of O₂ per minute per cell. Each bar represents the mean ± SD of three independent experiments. (C) Release of cytochrome cinto the cytosole in Neo, but not in Bcl-2 overexpressing cells. A representative experiment of three is shown.