Effect of trolox (A) and BA (B) on ▵ψ_m, ROS production, cardiolipin content, and of trolox on the activity of OXPHOS complex IV in NO-treated APO-S Jurkat cells. (A and B) Cells were incubated for 8 and 24 hours with NO donor GTN (0.2 mmol/L) alone, trolox (5 mmol/L) alone, BA (60 μmol/L) alone or with GTN together with either trolox or BA. Flow cytometry analysis for ▵ψ_m [DiOC₆(3)], ROS production (HE), and for cardiolipin concentration (NAO) was performed as described in the legend to Fig 2. (□), DiOC₆ (3)^low; (░), HE^high; (▪), NAO^low. (C) Cells were incubated with NO donor GTN (0.2 mmol/L) alone, trolox (5 mmol/L) alone, and with both agents for 8 hours. Activity of OXPHOS complex IV was measured as described in the legend to Fig 1. Each bar represents the mean ± SD of three independent experiments.